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Method and performance evaluation in a laboratory comparison test with algal growth inhibition test

By March 18, 2024No Comments

We are pleased to share the poster our colleague Patricia Marini presented at SETAC in 2023.

Title: “Method and performance evaluation in a laboratory comparison test:Algal growth inhibition test with time-variable exposures

The Algal Growth Inhibition Test according to OECD guideline 201 is an essential part of the ecotoxicological risk assessment of plant protection products. However, the test system is limited in different exposure scenarios and does not reliably reflect real environmental conditions due to the static single-exposure. The innovative test setup of a combination of invitro experimental data and the SAM-X in silico toxicokinetic/toxicodynamic (TK/TD) model for green-micro algae give a deeper insight into population effects and thus might refine future Tier 2C risk assessments for primary producers. Based on comments from EFSA on this test set up Crop Life Europe wanted to investigate the general robustness and reproducibility of two methods (semi-static and flow through) for the validation and calibration of the algae TK/TD models. As part of their laboratory comparison test, we performed algae growth inhibition tests as semi-static test designs and in flow-through bioreactors with multiple peaks of the test substance and time variable intervals between exposures. We present the feasibility and some exposure scenarios of the flow-through system using Raphidocelis subcapitata and an herbicide.