Metabolism
We investigate the fate and behaviour of your substance in Crops and the accumulation in Animals
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We provide in vitro and crop metabolism studies using radiolabelled (recommended) or unlabelled substances at IES. Our studies meet all main international criteria required for registration, with tests run according to OECD, EPA/OCSPP and JMAFF guidelines.
In vitro Testing
We offer a battery of in vitro metabolism assays that are used at different stages of the development process and when experiments with human volunteers are not possible.
- Metabolic stability determination
- Metabolic profiling and metabolite identification
- Comparative studies (cross-species comparisons between human and animals)
Test Systems
Cell cultures (e.g., hepatocytes) and subcellular fractions (e.g., liver microsomes and S9 fractions) from human and different animal species used in pivotal safety studies (e.g. rat, mouse, rabbit and dog).
Crops
Metabolism studies determine the behaviour of a test substance and the nature of residues in crop plants, so our customers can evaluate dietary exposure and the potential impact of their product on humans and animals.
- Metabolism in primary and rotational crops: OECD 501, OECD 502
- Plant Uptake Factor (PUF)/Transpiration Stream Concentration Factor (TSCF)
The PUF/TSCF model parameters represent the proportion of chemical absorbed into the plant via the roots, and thus removed from the soil. Experimental determination of the amount of a compound taken up by the plant is more and more often required for the environmental risk assessment
Bioaccumulation
We determine the potential for bioaccumulation in Hyalella and in fish. Fish species commonly used: zebrafish or rainbow trout.
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Bioaccumulation in fish: OECD 305
- Hyallela azteca Bioconcentration Test (HYBIT): OECD 321
Skin Penetration
We also investigate absorption of active substances and other toxicologically relevant compounds through the skin. The dermal penetration study uses excised rat or human skin and delivers the most relevant, translational data to clearly assess your compound’s behaviour on skin.
- Skin Absorption (in vitro method): OECD 428.
Human safety
These data can be compared with human data for risk assessment or between-species comparison requirements and utilized to design dermal ADME/toxicology studies.
